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Synonym(s):
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STEC;
O157:H7; E. coli
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Requisition Form
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G-22-(Food);
G-23; G-21 Path-Pathogen; MSA-FORM 50-1
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Test Description
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BAX®
Real-Time PCR Screen and/or conventional biochemical tests and enrichment to isolate
and identify E. coli O157:H7
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Pre-Approval Needed
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Regulatory
or IDCU agent
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Sample/Specimen Type for Testing
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- Food/milk
samples
- Environmental
Swabs
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Minimum Volume/Size Required
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- 100
grams (1/4 pound); or
- 500
grams (1.1 pounds) for beef trim, ground beef
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Storage/Preservation Prior to Shipping
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- Environmental
swabs must be collected on appropriate swabs used for environmental testing
(e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent).
- Place
food sample in sterile zip-lock or whirlpack bag or other sterile plastic
container.
- Store
on ice.
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Transport Medium
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N/A
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Specimen Labeling
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Sample
Identification must match submission form.
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Shipping and Specimen Handling Requirements
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- Ship samples on Ice and/or Ice Packs.
- Samples must be shipped for overnight delivery.
- Ship according to Dangerous Good Regulations,
IATA, and/or CFR 49.
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Method
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- BAX®
Real-Time PCR Screen
- FDA
Bacteriological Analytical Manual (BAM)- Chapter 4A
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Turn-around Time
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14
days
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Interferences/Limitations
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- The
sample should represent the outer surface as well as the interior of the
food.
- All
milk samples must be tested within 60 hours of collection.
- Environmental
swabs must be tested within 48 hours of collection.
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| Common Causes for Rejection
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- Not collected by a Registered Sanitarian
- Insufficient quantity
- Improperly stored
- Failure to meet shipping guidelines
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Additional Information
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N/A
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