Synonym(s):
|
STEC;
O157:H7; E. coli
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Requisition Form
|
G-22-(Food);
G-23; G-21 Path-Pathogen; MSA-FORM 50-1
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Test Description
|
BAX®
Real-Time PCR Screen and/or conventional biochemical tests and enrichment to isolate
and identify E. coli O157:H7
|
Pre-Approval Needed
|
Regulatory
or IDCU agent
|
Supplemental Information Required
|
N/A
|
Supplemental Form(s)
|
N/A
|
Performed on Specimens from (sources)
|
|
Sample/Specimen Type for Testing
|
- Food/milk
samples
- Environmental
Swabs
|
Minimum Volume/Size Required
|
- 100
grams (1/4 pound); or
- 500
grams (1.1 pounds) for beef trim, ground beef
|
Storage/Preservation Prior to Shipping
|
- Environmental
swabs must be collected on appropriate swabs used for environmental testing
(e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent).
- Place
food sample in sterile zip-lock or whirlpack bag or other sterile plastic
container.
- Store
on ice.
|
Transport Medium
|
N/A
|
Specimen Labeling
|
Sample
Identification must match submission form.
|
Shipping and Specimen Handling Requirements
|
- Ship samples on Ice and/or Ice Packs.
- Samples must be shipped for overnight delivery.
- Ship according to Dangerous Good Regulations,
IATA, and/or CFR 49.
|
Method
|
- BAX®
Real-Time PCR Screen
- FDA
Bacteriological Analytical Manual (BAM)- Chapter 4A
|
Turn-around Time
|
14
days
|
Interferences/Limitations
|
- The
sample should represent the outer surface as well as the interior of the
food.
- All
milk samples must be tested within 60 hours of collection.
- Environmental
swabs must be tested within 48 hours of collection.
|
Common Causes for Rejection
|
- Not collected by a Registered Sanitarian
- Insufficient quantity
- Improperly stored
- Failure to meet shipping guidelines
|
Additional Information
|
N/A
|