Laboratory Fee Schedule
Procedure: MCD0276A
CPT: 87143
| Synonym(s): |
Actino, Nocardia, Actinomadura |
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| Requisition Form |
G-MYCO |
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| Test Description |
Test for the identification of aerobic Actinomycete isolates, not Mycobacterium, referred to DSHS Laboratory |
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| Pre-Approval Needed |
N/A |
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| Supplemental Information Required |
N/A |
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| Supplemental Form(s) |
N/A |
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| Performed on Specimens from (sources) |
Pure isolate from human sources |
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| Sample/Specimen Type for Testing |
Aerobic Actinomycete pure isolate in liquid or on solid culture medium |
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| Minimum Volume/Size Required |
- Liquid culture - 2.0 mL
- Solid culture - Single mature colony
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| Storage/Preservation Prior to Shipping |
Ambient |
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| Transport Medium |
N/A |
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| Specimen Labeling |
- At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49
- Category B shipping applies for unidentified isolates
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| Method |
- High performance liquid chromatography (HPLC)
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| Turn-around Time |
HPLC is one test in a battery of tests that take 3 to 4 weeks for full identification. |
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| Interferences/Limitations |
- HPLC may only detect the predominant organism in a mixed culture.
- HPLC cannot definitively identify an aerobic Actinomycete.
- Additional testing methods are needed provide definitive identification.
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| Common Causes for Rejection |
- Cultures infested with mites
- Cultures broken in transit
- Missing identifiers on specimen or submission form
- Mismatch between specimen and/or submission form identifiers
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| Additional Information |
- Within one week of isolate receipt, and based on the HPLC analysis, the absence or presence of cell wall mycolic acids can be determined and/or a presumptive identification made based on mycolic acid pattern similarity to a given genus.
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