AFBhplc

Acid Fast Bacilli Isolate Identification by High 

Performance Liquid Chromatography (HPLC)   

Laboratory Fee Schedule

Procedure: MAC0052A

CPT: 87143

 

Acid Fast Bacilli Isolate Identification by High Performance Liquid Chromatography (HPLC)

Synonym(s):

HPLC Identification of clinical isolates

Requisition Form

G-MYCO

Test Description

Mycolic acid analysis identification of mycobacterial isolates cultured from clinical specimens in DSHS Laboratory

Pre-Approval Needed

N/A

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)

Pure isolate from human sources

Sample/Specimen Type for Testing

Mycobacterium species recovered by DSHS laboratory in liquid or on solid culture medium

Minimum Volume/Size Required

  • Liquid culture- 2.0 mL
  • Solid culture- Single mature colony

Storage/Preservation Prior to Shipping

N/A

Transport Medium

N/A

Specimen Labeling

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)

  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

N/A

Method

High performance liquid chromatography

Turn-around Time

2 days

Interferences/Limitations

  • HPLC may only detect the predominant organism in a mixed culture.
  • Liquid cultures must be instrument positive in order to analyze by HPLC.
  • Additional testing methods may need to be used to provide definitive identification

Common Causes for Rejection

N/A

Additional Information

  • This test is used in-house to identify Mycobacterium species cultured from submitted clinical specimens.