Acid Fast Bacilli Isolate Identification by High Performance Liquid Chromatography (HPLC)

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Acid Fast Bacilli Isolate Identification by High Performance Liquid Chromatography (HPLC)

Laboratory Fee Schedule
Procedure: MAC0052A
CPT: 87143

Synonym(s):HPLC Identification of clinical isolates
Requisition FormG-MYCO
Test DescriptionMycolic acid analysis identification of mycobacterial isolates cultured from clinical specimens in DSHS Laboratory
Pre-Approval NeededN/A
Supplemental Information RequiredN/A
Supplemental Form(s)N/A
Performed on Specimens from (sources)Pure isolate from human sources
Sample/Specimen Type for TestingMycobacterium species recovered by DSHS laboratory in liquid or on solid culture medium.
Minimum Volume/Size Required
  • Liquid culture- 2.0 mL
  • Solid culture- Single mature colony
Storage/Preservation Prior to ShippingN/A
Transport MediumN/A
Specimen Labeling
  • At least two patient identifiers are required on specimen (e.g., patient full name, date of birth, medical record number).
  • Three patient identifiers are preferred.
  • Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
  • Additional DSHS Laboratory specimen labeling guidelines available online here.
Shipping and Specimen Handling RequirementsN/A
MethodHigh performance liquid chromatography (HPLC)
Turn-around Time2 days
Interferences/Limitations
  • HPLC may only detect the predominant organism in a mixed culture. 
  • Additional testing methods may need to be used to provide definitive identification.
Common Causes for RejectionN/A
Additional Information
  • This test is used in-house to identify Mycobacterium species cultured from submitted clinical specimens.