Arbovirus IgM EIA, St. Louis Encephalitis and West Nile

Laboratory Fee Schedule

Procedure: MZZ0085A

CPT: 86653, 86789


Arbovirus IgM EIA, St. Louis Encephalitis and West Nile


Synonym(s): Capture Enzyme Immunoassay for the Detection of IgM Antibodies to West Nile and St. Louis Encephalitis Viruses
Requisition Form G-2A
Test Description Capture Enzyme Immunoassay (EIA) to detect the presence of specific IgM antibodies
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Serum 1.0 ml
  • Cerebral Spinal Fluid (CSF) 400 uL
Minimum Volume/Size Required 1.0 mL
Storage/Preservation Prior to Shipping
  • Specimen should be frozen at -20°C or colder
  • May be stored between 2°C to 8°C if it will be tested within two days.
Transport Medium N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions.
  • Triple contain in accordance with federal shipping regulations for infectious agents. Additional forms & resources
Method Capture EIA
Turn-around Time 8 working days
Interferences/Limitations Cross-reactions can occur between flaviviruses. Specific IgG can compete with IgM and may result in a false negative. Rheumatoid factor in presence of specific IgG may result in a false positive.
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form, and/or incomplete or missing submission form.
Additional Information Convalescent serum specimen should be tested if an early acute CSF or serum is negative. For most, IgM is detectable 8 days post-onset of symptoms and can persist for at least 45 days and up to 90 days.