PCR: Brucella species

Laboratory Fee Schedule
Procedure #: BTA0025
CPT: 87798

 
 
Synonym(s):          Brucella species (abortus, canis, melitensis, suis) Real-Time PCR, Brucellosis Real-Time PCR
Requisition FormG-27A
Test DescriptionDetection of Brucella species DNA by Real-Time PCR
Pre-Approval NeededMust notify BioThreat Team prior to sample submission: (512) 689-5537.
Supplemental Information RequiredN/A
Supplemental Form(s)N/A
Performed on Specimens from (sources)
  • Human
  • Animal
  • Environmental
Sample/Specimen Type for Testing
  • Culture isolates
  • Whole blood
  • Serum
  • Environmental specimens
  • Food (fresh/frozen milk, cheese)
  • Animal blood and tissue
Minimum Volume/Size Required

Culture isolates: 1 agar slant or plate

Clinical samples: 1 mL

Storage/Preservation Prior to Shipping

Culture isolates

  • 2-8°C (cold) 

Environmental samples 

  • Letters, powders, liquids, plant material
    • Ambient
  • Milk, cheese, animal blood
    • 2-8°C (cold)
  • Animal tissues
    • -20°C (frozen)
Transport MediumN/A
Specimen Labeling

Human

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.

Non-Human or Environmental

  • Sample identification must match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions.
  • Triple contained in accordance with federal shipping regulations for infectious agents. 
  • See also Emergency Preparedness resources online.

Notify BioThreat Team prior to sample submission: (512) 689-5537.

MethodReal-Time PCR
Turn-around TimePreliminary result 1 day after receipt, final result 1- 21 days
Interferences/LimitationsImproper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form
Additional InformationMay be paired with Definitive ID: Brucella species