Brucella spp. PCR

Laboratory Fee Schedule
Procedure #: BTA0025
CPT: 87798

 

Synonym(s):          

Brucella species (abortus, canis, melitensis, suis) PCR,  Brucellosis PCR

Requisition Form

G-27A

Test Description

Detection of  Brucella species DNA by PCR

Pre-Approval Needed

Must notify BioThreat Team prior to sample submission: (512) 689-5537.

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)
  • Non-Human (Bacterial)
  • Human
  • Environmental

Sample/Specimen Type for Testing
  • Culture isolates
  • Whole blood
  • Serum
  • Environmental specimens

Minimum Volume/Size Required

Culture isolates: 1 agar slant or plate

Clinical samples: 1 mL

Storage/Preservation Prior to Shipping

Culture isolates

  • 2-8°C (cold) 

Environmental samples 

  • Letters, powders, liquids, plant material
    • Ambient
  • Milk, cheese, animal blood
    • 2-8°C (cold)
  • Animal tissues
    • -20°C (frozen)

Transport Medium

N/A

Specimen Labeling

Human

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.

Non-Human or Environmental

  • Sample identification must match submission form.

Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions.
  • Triple contained in accordance with federal shipping regulations for infectious agents. 
  • See also Emergency Preparedness resources online.

Notify BioThreat Team prior to sample submission: (512) 689-5537.

Method

PCR

Turn-around Time

Preliminary result 1 day after receipt, final result 1- 21 days

Interferences/Limitations

Improper storage or shipping temperatures may limit organism recovery.

Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form

Additional Information

May be paired with Definitive ID: Brucella species