Definitive ID: Brucella species

Laboratory Fee Schedule
Procedure #: BTA0001
CPT: 87077

Synonym(s):                                       Brucellosis, Brucella abortus, Brucella canis, Brucella melitensis, Brucella suis
Requisition Form G-27A
Test Description Culture Identification for Brucella species.
Pre-Approval Needed Must notify BioThreat Team prior to sample submission: (512) 689-5537.
Supplemental Information Required N/A
Supplemental Form(s) N/A

Performed on Specimens from (sources)

  • Non-Human (Bacterial)
  • Human
  • Environmental

Sample/Specimen Type for Testing

  • Culture isolates
  • Tissues
  • Environmental/nonclinical sample
Minimum Volume/Size Required

Culture isolates

  • 1 agar slant or plate

Clinical samples

  • 1 mL


  • 50 grams

Animal tissues

  • 2 grams (1 cm3)
Storage/Preservation Prior to Shipping

Culture isolates

  • Ambient
  • Tissues
    • -20°C (frozen)

Environmental samples

  • Letters, powders, liquids, plant material
    • Ambient
  • Milk, cheese, animal blood.
    • 2-8°C (cold)
  • Animal tissues
    • -20°C (frozen)
Transport Medium N/A
Specimen Labeling


•    Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
•    Identifiers on specimen must exactly match submission form.

Non-Human or Environmental

•    Sample identification must match submission form.

Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

  • Handled as infectious agent using universal precautions.

  • Triple contained in accordance with federal shipping regulations for infectious agents.

  • See also Emergency Preparedness resources online.

  • Notify BioThreat Team prior to sample submission: (512) 689-5537.

Method Conventional biochemical methods
Turn-around Time Preliminary results 1 day after receipt, final results 1 - 21 days.
Interferences/Limitations Material must be viable. Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form
Additional Information May be paired with PCR: Brucella species.