Dengue IgM

Laboratory Fee Schedule

Procedure: MAM0086A

CPT: 86790

Synonym(s): Arbovirus, Flavivirus
Requisition Form G-2V
Test Description IgM capture ELISA is for the qualitative detection of IgM antibodies to Dengue virus in serum for the presumptive diagnosis of Dengue fever. 
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing Serum collected in Serum Seperator tubes (SST) or red top tubes. 
Minimum Volume/Size Required 1.0 mL.
Storage/Preservation Prior to Shipping

Serum separated from the clot may be stored at 2°C to 8°Cup to 48 hours, but then must be frozen at -20°C or colder until used.                        

Transport Medium N/A  
Specimen Labeling
  • At least two patient-specific identifiers required (e.g., patient full name, date of birth, Medical Record number)
  • Three patient identifiers are preferred.
  • Identifiers on specimen must exactly match submission form.
  • Additional DSHS Laboratory specimen labeling guidelines available online here
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions. 
  • Triple contain in accordance with federal shipping regulations for infectious agents. 
    Additional forms & resources
  • If specimens will arrive at the lab within 48 hours of collection, refrigerate at 2°C - 8°and ship overnight on cold packs.
  • If specimens will arrive at the lab more than 48 hours after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.
  • Additional DSHS Laboratory specimen shipping guidelines online here.
Method Enzyme-Linked Immunosorbent Assay (ELISA)
Turn-around Time

7 working days

  • Cross-reactivity with other flaviviruses is very common. Sera from patients infected with Japanese Encephalitis, Wet Nile, and/or Saint Louis viruses may give false positive results. 
  • Repeated freezing and thawing of samples can cause antibody deterioration. 
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired collection container
  • Discrepancies between specimen label and submission form
  • Incomplete, illegible, or missing submission form.
Additional Information
  • Reactive samples will be forwarded to CDC for confirmatory testing by Plague Reduction Neutralization Test (PRNT).
  • Minimum volume for CDC confirmation testing 0.5 mL.