| Synonym(s): | Arbovirus, Flavivirus | 
	| Requisition Form | G-2V | 
	| Test Description | IgM capture ELISA is for the qualitative detection of IgM antibodies to Dengue virus in serum for the presumptive diagnosis of Dengue fever. | 
	| Pre-Approval Needed | N/A | 
	| Supplemental Information Required | N/A | 
	| Supplemental Form(s) | N/A | 
	| Performed on Specimens from (sources) | Human | 
	| Sample/Specimen Type for Testing | Serum collected in Serum Separator tubes (SST) or red top tubes. | 
	| Minimum Volume/Size Required | 1.0 mL. | 
	| Storage/Preservation Prior to Shipping | Serum separated from the clot may be stored at 2°C to 8°Cup to 48 hours, but then must be frozen at -20°C or colder until used.                         | 
	| Transport Medium | N/A | 
	| Specimen Labeling | 
	At least two patient-specific identifiers required (e.g., patient full name, date of birth, Medical Record number)Three patient identifiers are preferred.Identifiers on specimen must exactly match submission form.Additional DSHS Laboratory specimen labeling guidelines available online here.  | 
	| Shipping and Specimen Handling Requirements | 
	Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.Handle as infectious agent using universal precautions. Triple contain in accordance with federal shipping regulations for infectious agents. Additional forms & resources
If specimens will arrive at the lab within 48 hours of collection, refrigerate at 2°C - 8°and ship overnight on cold packs.If specimens will arrive at the lab more than 48 hours after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.Additional DSHS Laboratory specimen shipping guidelines online here. | 
	| Method | Enzyme-Linked Immunosorbent Assay (ELISA) | 
	| Turn-around Time | 7 working days | 
	| Interferences/Limitations | 
	Cross-reactivity with other flaviviruses is very common. Sera from patients infected with Japanese Encephalitis, Wet Nile, and/or Saint Louis viruses may give false positive results. Repeated freezing and thawing of samples can cause antibody deterioration.  | 
	| Common Causes for Rejection | 
	Insufficient quantityUnacceptable specimen type or sourceImproper shipping conditionsExpired collection containerDiscrepancies between specimen label and submission formIncomplete, illegible, or missing submission form. | 
	| Additional Information | 
	Reactive samples will be forwarded to CDC for confirmatory testing by Plague Reduction Neutralization Test (PRNT).Minimum volume for CDC confirmation testing 0.5 mL. |