| Synonym(s): | 
	Arbovirus, Flavivirus | 
	| Requisition Form | 
	G-2V | 
	| Test Description | 
	IgM capture ELISA is for the qualitative detection of IgM antibodies to Dengue virus in serum for the presumptive diagnosis of Dengue fever.  | 
	| Pre-Approval Needed | 
	N/A | 
	| Supplemental Information Required | 
	N/A | 
	| Supplemental Form(s) | 
	N/A | 
	| Performed on Specimens from (sources) | 
	Human | 
	| Sample/Specimen Type for Testing | 
	Serum collected in Serum Separator tubes (SST) or red top tubes.  | 
	| Minimum Volume/Size Required | 
	1.0 mL. | 
	| Storage/Preservation Prior to Shipping | 
	
	 Serum separated from the clot may be stored at 2°C to 8°Cup to 48 hours, but then must be frozen at -20°C or colder until used.                         
	 | 
	| Transport Medium | 
	N/A   | 
	| Specimen Labeling | 
	
	
	- At least two patient-specific identifiers required (e.g., patient full name, date of birth, Medical Record number)
 
	- Three patient identifiers are preferred.
 
	- Identifiers on specimen must exactly match submission form.
 
	- Additional DSHS Laboratory specimen labeling guidelines available online here. 
 
	 
	 | 
	| Shipping and Specimen Handling Requirements | 
	
	
	- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
 
	- Handle as infectious agent using universal precautions. 
 
	- Triple contain in accordance with federal shipping regulations for infectious agents. 
 
	Additional forms & resources 
	- If specimens will arrive at the lab within 48 hours of collection, refrigerate at 2°C - 8°and ship overnight on cold packs.
 
	- If specimens will arrive at the lab more than 48 hours after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.
 
	- Additional DSHS Laboratory specimen shipping guidelines online here.
 
	 
	 | 
	| Method | 
	Enzyme-Linked Immunosorbent Assay (ELISA) | 
	| Turn-around Time | 
	
	 7 working days 
	 | 
	| Interferences/Limitations | 
	
	
	- Cross-reactivity with other flaviviruses is very common. Sera from patients infected with Japanese Encephalitis, Wet Nile, and/or Saint Louis viruses may give false positive results. 
 
	- Repeated freezing and thawing of samples can cause antibody deterioration. 
 
	 
	 | 
	| Common Causes for Rejection | 
	
	
	- Insufficient quantity
 
	- Unacceptable specimen type or source
 
	- Improper shipping conditions
 
	- Expired collection container
 
	- Discrepancies between specimen label and submission form
 
	- Incomplete, illegible, or missing submission form.
 
	 
	 | 
	| Additional Information | 
	
	
	- Reactive samples will be forwarded to CDC for confirmatory testing by Plague Reduction Neutralization Test (PRNT).
 
	- Minimum volume for CDC confirmation testing 0.5 mL.
 
	 
	 |