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PCR: Francisella tularensis

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512-776-7318
Fax
512-776-7294

Specimen Receiving
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Phone: 512-776-7598

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PCR: Francisella tularensis

Laboratory Fee Schedule
Procedure #: BTA0028
CPT: 87798

Synonym(s): Francisella tularensis, francisella Tularemia, Rabbit fever
Requisition Form G-27A.
Test Description Detection of Francisella tularensis DNA by Real-time PCR
Pre-Approval Needed Must notify BioThreat Team prior to sample submission: (512) 689-5537.
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources)
  • Human
  • Environmental
Sample/Specimen Type for Testing
  • Pure culture isolate
  • Blood, Serum
  • Aspirate
  • Bone Marrow
  • Respiratory Secretions
Minimum Volume/Size Required

Culture isolates

  • 1 plate or slant
  • 1 ml for fluids

Liquids

  • 1 ml for

Solids

  • 2 grams
Storage/Preservation Prior to Shipping

Culture isolates

  • ambient,

Clinical samples

  • 2-8°C (cold), keep tissue moist
Transport Medium N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions.
  • Triple contained in accordance with federal shipping regulations for infectious agents.
  • See also Emergency Preparedness resources online.
  • Notify BioThreat Team prior to sample submission: (512) 689-5537
Method Real-time PCR
Turn-around Time Preliminary result 1 day after receipt, final result 21 days
Interferences/Limitations Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form
Additional Information May be paired with Definitive Identification: Francisella tularensis.