Gram-positive rod identification – Culture
Laboratory Fee Schedule
Procedure #: MAA0033A
CPT: 87077
| Synonym(s): | Gram positive non-spore forming rod, Listeria. |
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| Requisition Form | G-2B |
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| Test Description | Identification of gram-positive rods from reference culture |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Pure isolate |
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| Minimum Volume/Size Required | At least one viable colony |
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| Storage/Preservation Prior to Shipping | Maintain isolates at room temperature or cold at 2°C to 8°C. |
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| Transport Medium | Appropriate medium that supports growth |
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| Specimen Labeling | - At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g. abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Additional DSHS Laboratory specimen shipping guidelines online here.
- Ship overnight on cold packs if specimens are refrigerated.
- Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
- Do not ship specimens for holiday or weekend delivery.
- Ensure media used to isolate organisms are not expired.
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| Method | Bacterial culture, MALDI-TOF (Mass spectrometry) |
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| Turn-around Time | 2-21 days |
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| Interferences/Limitations | - Isolate must be viable.
- Improper storage or shipping temperatures
- Limit organism recovery.
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| Common Causes for Rejection | - Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
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| Additional Information | N/A |
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