Please refer to the Microbiology Unit Notices Page for information about a temporary change in hantavirus testing at the DSHS Laboratory.
Laboratory Fee Schedule
Procedure: MBC0198A
CPT: 86790
| Synonym(s): | Sin Nombre Virus, Andes Virus, Hantavirus Pulmonary Syndrome (HPS), New World Hantavirus |
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| Requisition Form | G-2A |
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| Test Description | Enzyme Immunoassay panel for the detection of IgG and IgM antibodies against the Sin Nombre Virus (Hantavirus). |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Serum |
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| Minimum Volume/Size Required | 500 µL |
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| Storage/Preservation Prior to Shipping | - Store serum at 2°C to 8°C for up to 7 days.
- For storage longer than 7 days, store at -20°C or below
- Diluted samples should be incubated at the appropriate temperature within one working day.
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| Transport Medium | N/A |
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| Specimen Labeling | - Two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations for infectious agents.
- If specimens will arrive at the Lab within 7 days of collection, refrigerate at 2°C –8°C and ship overnight on cold packs.
- If specimens will arrive at the Lab more than 7 days after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
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| Method | Enzyme-Linked Immunosorbent Assay (ELISA) |
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| Turn-around Time | 3 working days |
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| Interferences/Limitations | - Assay results are intended solely as an aid to diagnosis and should be interpreted in conjunction with the patient’s clinical presentation, epidemiologic exposure history, and other relevant laboratory and diagnostic findings.
- Negative results may reflect testing performed during the acute phase of illness prior to development of detectable antibody levels. If laboratory results are inconsistent with clinical findings, submission of a follow-up specimen is recommended to assist with interpretation.
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| Common Causes for Rejection | - Insufficient specimen volume.
- Unacceptable specimen type or source.
- Improper shipping conditions.
- Expired media or collection container.
- Expired reagents/media/collection container.
- Discrepancies between G-2A submission form and specimen label.
- Missing/incomplete/illegible submission form or label.
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| Additional Information | - Specimens positive for hantavirus IgM antibodies will be forwarded to CDC for confirmatory testing.
- The minimum volume for CDC confirmation testing is 100µL.
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