Norovirus Detection, PCR

Laboratory Fee Schedule
Procedure:  MZZ0450A
CPT: 87798

Synonym(s):Norwalk-like virus, stool
Requisition FormG-2B
Test DescriptionPCR test designed to detect the presence of RNA from human noroviruses and the Norwalk-like virus genera
Pre-Approval NeededN/A
Supplemental Information RequiredN/A
Supplemental Form(s)N/A  
Performed on Specimens from (sources)Human
Sample/Specimen Type for TestingStool (raw or in Cary-Blair transport medium)
Minimum Volume/Size Required
  • 500 µL if stool is loose, liquid or in Cary-Blair medium
  •  A pea-sized amount(~0.2g) of raw stool
Storage/Preservation Prior to Shipping
  • Store raw stool specimens between 2°C–8°C on frozen ice packs, or frozen on dry ice.
  • Ship raw stool in a clean, dry, sealable container.
  • Store stool in Cary-Blair medium either cold between 2°C–8°C, or at ambient temperature.  
  • Do not freeze specimens in Cary-Blair medium.
Transport MediumN/A
Specimen Labeling
  • At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
  • Three unique patient identifiers are preferred.
  • Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
  • Additional DSHS Laboratory specimen labeling guidelines available online here.
Shipping and Specimen Handling Requirements
  • Raw stool: Ship refrigerated at 2°C–8°C on frozen cold packs or frozen on dry ice for overnight delivery.
  • Cary-Blair stool specimens should be prepared using manufacturer-provided guidance.
  • Do not freeze stool specimens in Cary-Blair.
  • Use multiple frozen cold packs when shipping cold.
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Category B Biological Substance, UN3373 shipping requirements apply.
  • Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
  • Additional DSHS Laboratory specimen shipping guidelines online here.
MethodReal-time PCR
Turn-around Time48 business hours from receipt of specimen
Interferences/LimitationsHeme (from hemoglobin) interferes with PCR in this assay.
Common Causes for Rejection
  • insufficient specimen volume
  • mismatch(es) between patient identifiers on G-2B submission form and specimen label
  • bloody stool
  • dried stool
  • improper storage conditions
  • raw stool specimens received at ambient temperature
  • Cary-Blair specimens received frozen
  • failure to meet specimen submission or shipping requirements
  • missing/incomplete/illegible submission form
Additional InformationPCR test may be paired with bacteriological culture.