Vibrio cholerae Serotyping

Laboratory Fee Schedule
Procedure #:  MZZ0400A
CPT:  87147

Synonym(s): Cholera, 01, Inaba, Ogawa, V. cholerae
Requisition FormG-2B
Test DescriptionSerotyping of Vibrio cholerae from reference culture
Pre-Approval NeededN/A
Supplemental Information RequiredN/A
Supplemental Form(s)N/A
Performed on Specimens from (sources)Human
Sample/Specimen Type for TestingPure isolate
Minimum Volume/Size RequiredAt least one viable colony.
Storage/Preservation Prior to ShippingKeep isolates at room temperature (15°C to 25°C)
Transport MediumAppropriate medium that supports growth
Specimen Labeling
  • At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
  • Three unique patient identifiers are preferred. 
  • Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
  • Additional DSHS Laboratory specimen labeling guidelines available online here.
     
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Category B Biological Substance, UN3373 shipping requirements apply.
  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements. 
  • Ship overnight to Laboratory at room temperature.
  • Do not refrigerate
  • Ship specimens overnight Monday–Thursday to avoid weekend deliveries. 
  • Do not ship specimens for holiday or weekend delivery. 
  • Ensure transport media are not expired.
  • Additional DSHS Laboratory specimen shipping guidelines online here.
     
MethodConventional serotyping O1, Inaba, Ogawa 
Turn-around Time4-14 days.
Interferences/Limitations
  • Isolate must be viable. 
  • Improper storage or shipping temperatures limit organism recovery.
Common Causes for Rejection
  • insufficient quantity
  • unacceptable specimen type or source
  • improper shipping conditions
  • expired media or collection container
  • discrepancies between specimen label and submission form
  • incomplete or missing submission form
Additional InformationSpecimens may be submitted to CDC for further characterization.