| Requisition Form |
G-2B |
| Test Description |
Antimicrobial susceptibility testing by ETest gradient strip for Azithromycin, Cefixime, Ceftriaxone, and Ciprofloxacin for suspected drug resistant Neisseria gonorrhoeae. |
| Pre-Approval Needed |
NA |
| Supplemental Information Required |
Provide summary of treatment/testing.
Provide most recent international or interstate travel within 1 month prior to specimen collection. Attach to submission form or write on reverse side of submission form.
|
| Supplemental Form(s) |
NA |
| Performed on Specimens from (sources) |
urethra, throat, oropharynx, rectum,
vagina, cervix, conjunctiva
|
| Sample/Specimen Type for Testing |
- Pure Isolate
- Clinical Sample
|
| Minimum Volume/Size Required |
- Pure Isolate: At least one viable colony
- Clinical: At least one viable colony on transport media such as Copan ESwab
|
| Storage/Preservation Prior to Shipping |
- Pure Isolate: should be grown on a non-selective medium (Chocolate II or GC base + 1% growth supplements) and incubated for 16-18 hours at 35-37°C in a 4-6% CO2-enriched atmosphere.
- Pure isolate alternative: Bacterial culture should be resuspended (≥4 McFarland) in trypticase soy broth (TSB) with 15-20% glycerol and freeze immediately at -70°C.
- Clinical sample: ESwabs: store in transport media at room temperature.
|
| Transport Medium |
Copan ESwab Transport for clinical samples available from DSHS upon request.
Acceptable transport medium: Copan ESwab,
InTray GC, Jembec plates, Transgrow bottle. |
| Specimen Labeling |
- Two patient-specific identifiers required (e.g., patient full name, date of birth, medical record number)
- Identifiers on specimen must exactly match submission form.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Ship isolate growth and clinical specimens overnight at ambient temperature Monday-Thursday to avoid weekend deliveries.
- Ship specimens in trypticase soy broth with glycerol which are frozen at -70°C on dry ice overnight Monday-Thursday to avoid weekend deliveries. Dry ice shippers and dry ice are not provided by DSHS.
|
| Method |
ETest Gradient strip AST |
| Turn-around Time |
10 days |
| Interferences/Limitations |
- Specimen must be viable for testing.
|
| Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
- Specimens not suspected of treatment failure
|
| Additional Information |
- To order the test choose “Neisseria” and write “GC AST test”
- Identification confirmation and beta-lactamase testing will also be performed.
|