Laboratory Fee Schedule
Procedure #: MPS0008A
CPT: 86753
| Synonym(s): | Trypanosoma cruzi, T. cruzi, Chagas Screen |
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| Requisition Form | G-2A |
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| Test Description | Qualitative detection of IgG antibodies against Trypanosoma cruzi, the causative agent of Chagas disease, using two separate indirect Enzyme-Linked Immunosorbent Assays (ELISA). |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Preferred Specimen: - Serum collected in Serum Separator tubes (SST) or red top tubes
Alternative Specimen: - Plasma collected in purple top tubes with EDTA
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| Minimum Volume/Size Required | 1 mL of serum or plasma |
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| Storage/Preservation Prior to Shipping | - Store at 2°C to 8°C for up to 7 days.
- Store at -20°C or colder for up to 60 days.
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| Transport Medium | N/A |
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| Specimen Labeling | - At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g. abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions.
- Triple contained per Federal shipping regulations for infectious agents.
- Refrigerate at 2°C–8°C and ship overnight on cold packs if specimens will arrive at the Lab within 7 days of collection.
- Freeze in secondary container at -20°C or colder and ship overnight on dry ice if specimens will arrive at the Lab more than 7 days after date of collection.
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| Method | Enzyme-Linked Immunosorbent Assay (ELISA) |
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| Turn-around Time | 7 working days |
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| Interferences/Limitations | - Hemolysis, hyperlipemia or other causes of turbidity may cause erroneous results.
- Heat-inactivated samples may yield false-positive results.
- A negative result does not exclude the possibility of exposure or infection by T. cruzi.
- Antibodies produced in response to other typanosomal infections may cross react with the
T. cruzi antigen preparation used in one of the ELISA assays.
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| Common Causes for Rejection | - insufficient specimen volume
- unacceptable specimen type or source
- improper shipping conditions
- expired media or collection container
- discrepancies between specimen label and submission form
- failure to meet specimen submission or shipping requirements
- missing/incomplete/illegible submission form
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| Additional Information | - All inconclusive and equivocal serum specimens will be forwarded to CDC for additional testing.
- Serum specimens are preferred; CDC does not accept plasma specimens for Chagas testing.
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