Chagas IgG

Laboratory Fee Schedule
Procedure #: MPS0008A 
CPT: 86753 

Synonym(s): Trypanosoma cruzi IgG Antibody Immunoassay 
Chagas Serologic Screen
Requisition Form G-2A
Test Description Qualitative detection of IgG antibodies against Trypanosoma cruzi, the causative agent of Chagas disease, using two separate indirect Enzyme-Linked Immunosorbent Assays (ELISA).
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Preferred Specimen:
    • Serum collected in Serum Separator tubes (SST) or red top tubes
  • Alternative Specimen: 
    • Plasma collected in purple top tubes with EDTA
Minimum Volume/Size Required 1 mL of serum or plasma
Storage/Preservation Prior to Shipping
  • Store at 2°C to 8°C for up to 7 days
  • Store at -20°C or colder for up to 60 days
Transport Medium N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions.
  • Triple contained in accordance with federal shipping regulations for infectious agents. Additional forms & resources
Method Indirect Enzyme-Linked Immunosorbent Assay (ELISA)
Turn-around Time 7 working days
  • Hemolysis, hyperlipemia or other causes of turbidity may cause erroneous results.  
  • Heat-inactivated samples may yield false-positive results.
  • A negative result does not exclude the possibility of exposure or infection by T. cruzi.
  • Antibodies produced in response to other typanosomal infections may cross react with the T. cruzi antigen preparation used in one of the ELISA assays.
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form
Additional Information
  • All Positive, Equivocal, and Inconclusive serum specimens will be forwarded to the CDC for confirmatory testing.  
  • Serum specimens preferred as the CDC does not accept plasma specimens for confirmatory testing.