Laboratory Fee Schedule
Procedure Number: MAA0025A
| Synonym(s): |
Listeriosis, culture |
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| Requisition Form |
G-2B |
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| Test Description |
Isolation of Listeria monocytogenes from clinical specimen (with reflex to definitive identification and whole genome sequencing (WGS) |
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| Pre-Approval Needed |
N/A |
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| Supplemental Information Required |
N/A |
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| Supplemental Form(s) |
N/A |
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| Performed on Specimens from (sources) |
- Human (stool culture for L. monocytogenes is not performed)
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| Sample/Specimen Type for Testing |
- Blood: Collect in red top, tiger top tube, or blood culture bottle
- CSF and amniotic fluid: collect in screw-top sterile container
- Tissue: collect by biopsy or autopsy procedures and place in screw top sterile container
- Placenta: collect specimen in screw-top sterile container
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| Minimum Volume/Size Required |
- Blood, CSF, amniotic fluid: 1ml
- Tissue, placenta: “pea-sized” or larger
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| Storage/Preservation Prior to Shipping |
Store refrigerated at 2–8°C until shipment. |
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| Transport Medium |
N/A |
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| Specimen Labeling |
- At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements |
- Ship refrigerated at 2–8°C on cold packs for overnight delivery to Laboratory.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contained in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen shipping guidelines online here
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| Method |
Culture |
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| Turn-around Time |
5–10 days |
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| Interferences/Limitations |
- Specimen should be taken from patient prior to antimicrobial therapy.
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| Common Causes for Rejection |
- Incorrect specimen collection or submission methods
- Mismatch(es) between patient identifiers on G-2B submission form and specimen label.
- Insufficient specimen growth
- Incorrect storage conditions
- Expired reagents/media/collection container
- Discrepancies between G-2B submission form and specimen label
- Missing/incomplete/illegible submission form
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| Additional Information |
N/A |
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