Laboratory Fee Schedule
Procedure Number: MAA0025A
| Synonym(s): | Listeriosis, culture |
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| Requisition Form | G-2B |
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| Test Description | Isolation of Listeria monocytogenes from clinical specimen (with reflex to definitive identification and whole genome sequencing (WGS) |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human (stool culture for L. monocytogenes is not performed) |
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| Sample/Specimen Type for Testing | - Blood: Collect in red top, tiger top tube, or blood culture bottle.
- CSF and amniotic fluid: collect in screw-top sterile container.
- Tissue: collect by biopsy or autopsy procedures and place in screw top sterile container.
- Placenta: collect specimen in screw-top sterile container.
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| Minimum Volume/Size Required | - Blood, CSF, amniotic fluid: 1ml
- Tissue, placenta: “pea-sized” or larger
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| Storage/Preservation Prior to Shipping | Store refrigerated at 2°C–8°C until shipment. |
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| Transport Medium | N/A |
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| Specimen Labeling | - At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship refrigerated at 2°C–8°C on cold packs for overnight delivery to Laboratory.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contained in accordance with federal shipping regulations.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Laboratory does not supply collection kits or shipping materials.
- Additional DSHS Laboratory specimen shipping guidelines online here.
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| Method | Culture |
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| Turn-around Time | 5–10 days |
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| Interferences/Limitations | - Specimen should be taken from patient prior to antimicrobial therapy.
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| Common Causes for Rejection | - Incorrect specimen collection or submission methods
- Incorrect storage conditions
- Expired reagents/media/collection container
- Discrepancies between G-2B submission form and specimen label
- Missing/incomplete/illegible submission form
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| Additional Information | N/A |
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